Document Type: Original Article
Introduction: Breast milk health is one of the most salient elements in feeding of premature infants. Generally in initial of breast feeding, different methods of disinfecting such as irrigation with usual detergents like water and soap are used to eliminate the probable bacteria. The necessity of the usage of such items has not been mentioned in some studies, and even at the time of comparison, different results have been obtained. Therefore we decided to study the importance of irrigation method in different ways of breast milk, whether manually or by pump, before and after irrigation, on mothers of low birth weight infants.
Method: This study is a kind of controlled and by chance trial which evaluates the results of samples collected from breasts of mothers of low birth weight infants. Sampling from breasts of these mothers was done on the basis of two ways: Manual with & without irrigation, pump expression with & without irrigation. Every sample was collected in a sterile container, and these containers were sent to microbial laboratory as soon as possible. Then in that place culture was done from every sample. If after 24 hours, there was no bacteria in plates , the plates were incubated again for another 24 hours. And after 48 hours if there was no growth of bacteria, negative result was recorded. After gathering the data, they were analyzed by statistical spss software.
Results: The results showed that the separated bacteria in samples collected manually, with & without irrigation, were consisted of: Staphylococcus-coagulase negative, Staphyloccus aureus, Enterococcus spp, Klebsiella pneumonia. In one sample of without irrigation and four samples of with irrigation, lack of growth of bacteria was observed. In pump expression samples, with & without irrigation, the separated bacteria were consisted of: Staphylococcus-coagulase negative, Staphyloccus aureus, Enterococcus spp, Klebsiella pneumonia, Enterobacter and Pseudomonas aeruginosa. Among the samples, in three samples of without irrigation and two samples of with irrigation, lack of growth of bacteria was observed.
Conclusion: The results and surveys showed that bacteria load in pump samples were much more than manual samples. Perhaps one of the reasons for this is the creation of a biofilm which is resistant against irrigation in the pump. The pump device also may follow completely the infecting rule and their disinfections.