Investigating the Rate of Group B Streptococcus in Below 3 Months Year Old Infants with Sepsis Clinical Symptoms Hospitalized in Ghaem Hospital of Mashhad

Document Type : Original Article


1 Neonatal research center, Faculty of medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Neonatal research center, Faculty of medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Pediatrician, Faculty of medicine, Mashhad University of Medi cal Sciences, Mashhad, Iran.

4 Microbiologist, Department of microbiology, Faculty of medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Neonatal research center, Mashhad University of Medical Sciences, Mashhad, Iran.


Introduction Group B streptococcus is the main reason of neonatal infection in developed countries and causes a widespread clinical indications. In developing countries such as Iran, its rate of appearance is not determined. With regard to colonization and the relative high epithelia of group B streptococcus, it is likely that the incidence of group B streptococcus in neonatal sepsis is so high.In the present study, we attempted to use the molecular methods to identify this bacterium and develop the culture environments as well.
Methods In the present study, a hundred below three months year old infants with sepsis hospitalized in ICU sector of Ghaem hospital for one year were studied since Khordad 1388 (June 2010). After getting consent from the infants’ parents, three blood samples of these patients in the sterile container with lid were transferred to the laboratory (two samples for culturing in normal environment and the other for PRC). All of the information was filled out by doctors in the incidence. SPSS 11.5 and descriptive-analytic tests were used for data analysis.
Results With regard to the high rate of anti-biotic consumption by mothers before delivery, it is necessary to use more sensitive methods like PCR to identify the Group B Streptococcus.
Conclusion Acceptable results and a growing survival rate was observed in this series of patients and we hope better results with improvements in  minimally invasive methods.


1.Tiskumara R, Fakharee SH, Liu CQ, Nuntnarumit P, Lui KM, Hammoud M, et al. Neonatal infections in Asia. Arch Dis Child Fetal Neonatal Ed. 2009 Mar;94(2):F144-8. Epub 2008 Sep 19.
2.Infection in newborn babies availabe at: http://www.about kids Pregnancy/ Infection-in-Newborn-Babies.aspx/ article ID= 7680&category ID=PG-nh4-10g. Accessed  Apr 18,2010.
3.Daley AJ, Isaacs D. Ten-year study on the effect of intrapartum antibiotic prophylaxis on early onset group B streptococcal and Escherichia coli neonatal sepsis in Australasia. Pediatr Infect Dis J. 2004 Jul;23(7):630-4.
4.Trends in perinatal group B streptococcal disease. 2009. MMWR.Available at: http:// mmwrhtml/mm5805a2.htm. Accessed Jul25,2010.
5.Shet A, Ferrieri P. Neonatal and maternal group B streptococcal infections: a copprehensive review. Indian J Med Res 2004; 141150.
6. Elsaid MF, Flamerzi AA, Bessisso MS, Elshafie SS. Acute bacterial meningitis in Qatar. Saudi Med J. 2006 Feb;27(2):198204.
7. Trotman H, Bell Y. Neonatal group B streptococcal infection at the University Hospital of the West Indies, Jamaica: a 10-year experience.Ann Trop Paediatr. 2006 Mar; 26(1):53-7.
8. Robinson TD, Kumar p,Cadichon  BS. Sepsis in the emergency department.CPEM 2008; 9:160-8.
9.Namavar Jahromi B, Poorarian S, Poorbarfehee S. The prevalence and adverse effects of group B streptococcal colonization during pregnancy. Arch Iran Med. 2008 Nov; 11(6):654-7.
10. Zamanzad B. Prevalence of  vaginal strep B carrier in pregnant women attending the hospital's maternity ward Shahre cord city. Journal of Shaeed Sdoughi University of Medical Sciences Yazd 2002, Jun; 10(3):       27-31.
11.Sarafrazi N, Mesdaghi nia E,Moniri R,Mosavi GA. Prevalence of group B beta-hemolytic Streptococcus of vaginal flora in pregnancy and its relation to early neonatal infections and infections during pregnancy. Feyz 2001Feb;4(18):22-7.
12.Rabiee S, Arab M, Yousefi Mashouf R. Epidemiologic Pattern of Vaginal colonization by group B streptococcus in pregnant women in Hamadan, central west of Iran. Iran J Med Sci 2006Jun; 31(2): 106-108.
13.Mosayebi Z, Movahedian AH, Moniri R. Profile of bacterial sepsis in neonates from Kashan in Iran. J Infect Dis Antimicrob Agents 2003; 20:97-102.
14.Amid MH.Cases of sepsis and meningitis in newborns hospitalized in Mofid Children's Hospital. Pejouhesh dar Pezeshki  2002; 26 (57): 1-63.
15.Fatehi I,Nourozy Z, Naseri M.Review of 82 cases of meningitis in infants under the 2 months during 14-year.TUMJ 1998;3(1):46-51.
16. Gharebaghi MM, Maamuri GA,Peirovifar A,Boskabadi H,Afshari JT. Immediate diognosis of early onset in premature newborns by measurment of cold C-Reactive protein and interlukin-6.IJMS 2007;132:217-21.
17.Kong F, Ma L, Gilbert GL. Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. J Med Microbiol. 2005 Dec;54(Pt 12):1133-8.
18. Jordan JA, Durso MB. Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis. J Mol Diagn. 2005 November; 7(5): 575–81
19. Golden SM, Stamilio DM, Faux BM, dela Cruz WP, Shoemaker CT, Blackmon CL,et al. Evaluation of a realtime fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood. Diagn Microbiol Infect Dis. 2004 Sep;50(1):7-13.
20. Deng JH, Yao KH, Hu HL, Yu SJ, Gao W, Fu LB,et al. Detection of group B streptococcus in the cases died of neonatal pneumonia. Zhonghua Er Ke Za Zhi. 2006 Nov;44(11):850-4.
21. Movahedian AH, Moniri R, Mosayebi Z. Bacterial culture of neonatal sepsis. Iranian J Publ Health 2006; 35:84-9.
22. Andersen J, Christensen R, Hertel J. Clinical features and epidemiology of septicaemia and meningitis in neonates due to Streptococcus agalactiae in Copenhagen County, Denmark: a 10 year survey from 1992 to 2001. Acta Paediatr. 2004 Oct; 93(10):1334-9.